Journal: PLoS ONE

Article Title: Exendin-4 Induces Cell Adhesion and Differentiation and Counteracts the Invasive Potential of Human Neuroblastoma Cells

doi: 10.1371/journal.pone.0071716

Figure Lengend Snippet: Representative experiment from three independent experiments on the invasive ability of SK-N-AS and SH-SY5Y cells after treatment with 0.3 µM exendin-4 (A); real-time RT-PCR analysis of the expression of MMP-9 and TIMP-1 in NB cells after treatment with 0.3 µM exendin-4 for 6 h. * = p<0.05 vs. related control (B). Size of the cell colonies grown in soft agar 7, 14 or 21 days after suspension, with or without (C = control) exendin-4. Data are reported as mean percentage vs. related control of three replicates. EXE = exendin-4; * = p<0.05 vs. related control; # = p<0.05 vs. C 7d; § = p<0.05 vs. C 14d (C).

Article Snippet: Primers and probe for uPAR (Hs00182181_m1), CXCR4 (Hs02330069_s1), microtubule-associated protein-2 MAP2 (Hs00159041_m1), Tau (Hs00902193_m1), Synaptophisin (Hs00300531_m1), Tissue inhibitor of metalloproteinases-1 (TIMP-1) (Hs99999139_m1), Matrix Metalloproteinase-9 (MMP-9) (Hs00957562_m1) were Assay-On-Demand products (Applied Biosystem).

Techniques: Quantitative RT-PCR, Expressing, Control, Suspension

Journal: Biomedical Reports

Article Title: Anti-allergic effect of the naturally-occurring conjugated linolenic acid isomer, jacaric acid, on the activated human mast cell line-1

doi: 10.3892/br.2015.517

Figure Lengend Snippet: Jacaric acid modulates the expression levels of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase-1 (TIMP-1) proteins in human mast cell line-1 (HMC-1) cells. HMC-1 cells were incubated with 1 (lane 2), 2 (lane 3) and 4 µM jacaric acid (lane 4) at 37°C for 72 h. Cells treated with 0.02% ethanol (lane 1) acted as the control. Subsequently, treated cells were further incubated with Iono and phorbol 12-myristate 13-acetate at 37°C for 6 h. (A) Protein expression levels of MMP-2, MMP-9 and TIMP-1 were assayed by western blotting with β-actin protein as an internal control. (B-D) The relative expression levels of MMP-9, MMP-2 and TIMP-1 proteins compared to β-actin were quantified. Results represent mean ± standard error. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: The membrane was first incubated with the following primary antibodies: Rabbit anti-MMP-2 (CST-4022S), anti-MMP-9 (CST-3852S), anti-tissue inhibitor of metalloproteinase-1 (TIMP-1) (CST-8946S) (Cell Signaling Technology, Inc., Danvers, MA, USA) and mouse anti-β-actin antibody (SC-A5316) (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), followed by incubation with mouse IgG horseradish peroxidase-conjugated secondary antibody (GE-NA931) or rabbit IgG horseradish peroxidase-conjugated secondary antibody (GE-NA934) (GE Healthcare, Buckinghamshire, UK and finally developed with the enhanced chemiluminescence reagent (Santa Cruz Biotechnology, Inc.).

Techniques: Expressing, Incubation, Control, Western Blot

Journal: International Journal of Medical Sciences

Article Title: Comparison the Prognostic Value of Galectin-3 and Serum Markers of Cardiac Extracellular Matrix Turnover in Patients with Chronic Systolic Heart Failure

doi: 10.7150/ijms.8083

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for prediction mortality. The area under the curve (AUC) of log MMP-2, log TIMP-1, log BNP, log PIIINP, log Gal-3, log PINP, and log MMP-9 were 0.786, 0.699, 0.636, 0.625, 0.607, 0.519, and 0.367, respectively. Abbreviations: BNP= brain natriuretic peptide; Gal-3= Galectin-3; HF=heart failure; LVEF= left ventricular ejection fraction; MMP = matrix metalloproteinase; PINP = type I amioterminal propeptide of procollagen; PIIINP = type III amioterminal propeptide of procollagen; TIMP = tissue inhibitor of metalloproteinase.

Article Snippet: TIMP-1 was measured by an ELISA kit (DTM100, R & D systems, Minneapolis, USA).

Techniques:

Journal: International Journal of Medical Sciences

Article Title: Comparison the Prognostic Value of Galectin-3 and Serum Markers of Cardiac Extracellular Matrix Turnover in Patients with Chronic Systolic Heart Failure

doi: 10.7150/ijms.8083

Figure Lengend Snippet: Kaplan-Meier analysis of cumulative rates of survival in HF patients with higher or lower levels of serum Gal-3 or cardiac ECM markers. The p value of Gal-3, PINP, PIIINP, TIMP-1, MMP-2, MMP-9, and BNP were 0.153, 0.708, 0.154, 0.028, 0.001, 0.501, and 0.483, respectively. Abbreviations: BNP= brain natriuretic peptide; ECM= Extracellular matrix; Gal-3= Galectin-3; HF=heart failure; LVEF= left ventricular ejection fraction; MMP = matrix metalloproteinase; PINP = type I amioterminal propeptide of procollagen; PIIINP = type III amioterminal propeptide of procollagen; TIMP = tissue inhibitor of metalloproteinase.

Article Snippet: TIMP-1 was measured by an ELISA kit (DTM100, R & D systems, Minneapolis, USA).

Techniques:

Journal: International Journal of Medical Sciences

Article Title: Comparison the Prognostic Value of Galectin-3 and Serum Markers of Cardiac Extracellular Matrix Turnover in Patients with Chronic Systolic Heart Failure

doi: 10.7150/ijms.8083

Figure Lengend Snippet: Kaplan-Meier analysis of cumulative rates of HF admission-free survival in HF patients with higher or lower levels of serum Gal-3 or cardiac ECM markers. The p value of Gal-3, PINP, PIIINP, TIMP-1, MMP-2, MMP-9, and BNP were 0.166, 0.624, 0.639, 0.085, <0.001, 0.624, and 0.684, respectively. Abbreviations: BNP= brain natriuretic peptide; ECM= Extracellular matrix; Gal-3= Galectin-3; HF=heart failure; MMP = matrix metalloproteinase; PINP = type I amioterminal propeptide of procollagen; PIIINP = type III amioterminal propeptide of procollagen; TIMP = tissue inhibitor of metalloproteinase.

Article Snippet: TIMP-1 was measured by an ELISA kit (DTM100, R & D systems, Minneapolis, USA).

Techniques: